威尼斯游戏大厅

杨崇林
教授/ clyang#ynu.edu.cn/细胞程序性死亡的调控机制

个人简历

         


1998年于北京大学获博士学位。

1998-1999年为意大利International school for advanced studies分子神经生物学实验室访问学者。

1999-2005年先后在University of Maryland School of Medicine和University of Colorado从事博士后研究。

2006年入选中国科学院 "百人计划",终期获评优秀。

2010年获国家杰出青年基金资助。

2013年被中国科协授予“全国优秀科技工作者”荣誉称号。

2016年威尼斯游戏大厅院长、生命科学研究中心主任、教授(二级)

2017年入选国家“百千万人才工程”。

FEBS Journal, Journal of Genetics and Genomic等国际国内外学术期刊编委。


主要研究方向

杨崇林研究组主要以秀丽线虫(Caenorhabditis elegans)为模式,运用正向和反向遗传、生化以及细胞生物学等综合手段,探索程序性细胞死亡的调控机制以及溶酶体和线粒体的调控机制。当前研究主要集中在以下三个方面:

1.细胞程序性死亡的遗传与分子调控机制

程序性细胞死亡是多细胞有机体生长和发育过程中最重要的生命现象之一,对组织和器官发育、生物体动态平衡的维持等起着决定性的作用。

2.溶酶体介导的细胞降解机制

溶酶体是细胞内物质降解的主要场所,我们利用重要模式生物-秀丽线虫作为强大的遗传工具,筛选和鉴定影响溶酶体功能和动态的必需调控因子。目前,通过多个正向遗传学筛选,我们已经获得多个表现溶酶体功能失调的突变体。

3.线粒体动态平衡的调控机制

我们将以线虫为模式,利用正、反向遗传学手段筛选并克隆参与线粒体代谢与细胞凋亡的新调控因子。我们将利用电镜分析、基因和功能表达分析、蛋白质亚细胞定位和动态分析,研究所克隆的新基因调控线粒体发生、形状维持的作用机制。

 

实验室成员和在读研究生

课题组成员:

张旭、尹秋媛、王鑫、李美娇、张洁、周合江、李婧琳

博士生:

马腾飞、李乐涛

研究生:

何用娟、赵珊、李倩

实验室网址:http://yanglab.genetics.ac.cn

 

杨崇林研究员2006-2020年发表文章(英文)


1.Qiuyuan Yin, Youli Jian, Meng Xu, Xiahe Huang, Niya Wang, Zhifang Liu, Qian Li, Jinglin Li, Hejiang Zhou, Lin Xu, Yingchun Wang, and Chonglin Yang*. (2020). CDK4/6 regulate lysosome biogenesis through TFEB/TFE3. Journal of Cell Biology. 219 (8): e201911036.


2.Ruofeng Tang, Xin Wang, Junxiang Zhou, Fengxia Zhang, Shan Zhao, Qiwen Gan, Liyuan Zhao, Fengyang Wang, Qian Zhang, Jie Zhang, Guodong Wang, Chonglin Yang*. (2020). Defective arginine metabolism impairs mitochondrial homeostasis in Caenorhabditis elegans. Journal of Genetics and Genomics. 47: 145-156.

 

3.Rui Miao, Meijiao Li, Qianqian Zhang, Chonglin Yang*, Xiaochen Wang. (2020). An ECM-to-nucleus signaling pathway activates lysosomes for C. elegans larval development. Developmental Cell. 52(1): 21-37. (As a cover article)


4.Qiwen Gan#,Xin Wang,Qian Zhang,Qiuyuan Yin#, Youli Jian,Yubing Liu,Nan Xuan,Jinglin Li#,Junxiang Zhou,Kai Liu,Yudong Jing,Xiaochen Wang,and Chonglin Yang*.The amino acid transporter SLC-36.1 cooperates with PtdIns3P 5-kinase to control phagocytic lysosome reformation.The Journal of Cell Biology.2019.doi: 10.1083/jcb.201901074.
 

5. Junxiang Zhou,Xin Wang,Min Wang,Yuwei Chang,Fengxia Zhang,Zhaonan Ban,Ruofeng Tang, Qiwen Gan, Shaohuan Wu, Ye Guo, Qian Zhang, Fengyang Wang, Liyuan Zhao, Yudong Jing, Wenfeng Qian,Guodong Wang,Weixiang Guo*,and Chonglin Yang*.The lysine catabolite saccharopine impairs development by disrupting mitochondrial homeostasis. The Journal of Cell Biology.2019,218(2): 580-597.

 

6. Min Wang#,Changyong Tang#,Ruxiao Xing,Xuezhao Liu,Yinghao Liu, Lei Wang, Chonglin Yang* and Weixiang Guo*.WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFβ signaling. Molecular Psychiatry. doi: 10.1038/s41380-018-0307-y.

 

7. Yang Li*, Yu Zhang, Qiwen Gan, Meng Xu, Xiao Ding, Guihua Tang, Jingjing Liang, Kai Liu, Xuezhao Liu, Xin Wang, Lingli Guo, Zhiyang Gao, Xiaojiang Hao*, Chonglin Yang*. (2018). C. elegans-based screen identifies lysosome-damaging alkaloids that induce STAT3-dependent lysosomal cell death. Protein & cell. 9(12):1013–1026.

 

8. Caitlin Morris, Olivia K. Foster, Simran Handa, Kimberly Peloza, Laura Voss, Hannah Somhegyi, Youli Jian, My Van Vo, Marie Harp, Fiona M. Rambo, Chonglin Yang, Greg J. Hermann*. (2018). Function and regulation of the Caenorhabditis elegans Rab32 family member GLO-1 in lysosome-related organelle biogenesis. PLoS Genetics. 14(11):e1007772.

 

9. Xinli Ma#,Kai Liu#,Jian Li,Huanhuan Li,Jun Li,Yingfang Liu, Chonglin Yang* and  Huanhuan Liang*. (2018). A non-canonical GTPase interaction enables ORP1L-Rab7-RILP complex formation and late endosome positioning. the Journal of Biological Chemistry. 293(36):14155-14164.

 

10. Kai Liu, Ruxiao Xing, Youli Jian, Zhiyang Gao, Xinli Ma, Xiaojuan Sun, Yang Li, Meng Xu, Xin Wang, Yudong Jing, Weixiang Guo and Chonglin Yang. (2017). WDR91 is a Rab7 effector required for neuronal development. The Journal of Cell Biology. 216(10): 3307-3321.

 

11. Jianhua Yin, Yaling Huang, Pengfei Guo, Siqi Hu, Sawako Yoshina, Nan Xuan, Qiwen Gan, Shohei Mitani, Chonglin Yang, Xiaochen Wang*. (2017). GOP-1 promotes apoptotic cell degradation by activating the small GTPase Rab2 in C. elegans. The Journal of Cell Biology. 216(6), 1775-1794.

 

12. Xuezhao Liu#, Yang Li#, Xin Wang,Ruxiao Xing,Kai Liu,Qiwen Gan,Changyong Tang,Zhiyang Gao,Youli Jian,Shouqing Luo,Weixiang Guo, and Chonglin Yang. (2017). The BEACH-containing protein WDR81 coordinates p62 and LC3C to promote aggrephagy. The Journal of Cell Biology. 216(5):1301-1320.

 

    13. Chonglin Yang*, Xiaochen Wang*. (2017). Cell Biology in China: Focusing on the Lysosome. Traffic. 18(6):348-357.

 

14. Shiya Cheng, Kai Liu, Chonglin Yang*, Xiaochen Wang*.(2017).Dissecting Phagocytic Removal of Apoptotic Cells in Caenorhabditis elegans. In Methods in Molecular Biology: Phagocytosis and Phagosomes ( Eds. Roberto Botelho ) Springer New York, 265-284. Print ISBN: 978-1-4939-6579-3.

 

15. Yuan Li, Baohui Chen, Wei Zou, Xin Wang,Yanwei Wu,Dongfeng Zhao, Yanan Sun, Yubing Liu, Lianwan Chen, Long Miao, Chonglin Yang, Xiaochen Wang*. (2016). The lysosomal membrane protein SCAV-3 maintains lysosome integrity and adult longevity. The Journal of Cell Biology 215(2), 167-185.

 

16. Yang Li#, Meng Xu#, Xiao Ding, Chen Yan, Zhiqin Song, Lianwan Chen, Xiahe Huang, XinWang,Youli Jian, Guihua Tang, Changyong Tang, Yingtong Di,Shuzhen Mu, Xuezhao Liu, Kai Liu, Ting Li,Yingchun Wang, Long Miao, Weixiang Guo, Xiaojiang Hao and Chonglin Yang. (2016). Protein kinase C controls lysosome biogenesis independently of mTORC1. Nature Cell Biology 18, 1065-1077.

(News and Views: Turn up the lysosome. Paul Saftig, Albert Haas. Nature Cell Biology 18, 1025-1027).

 

17. Xiaochen Wang*, Chonglin Yang*. (2016). Programmed cell death and clearance of cell corpses in Caenorhabditis elegans. Cellular and Molecular Life Sciences 73, 2221-2236.

 

18. Kai Liu#, Youli Jian#,Xiaojuan Sun#,Chengkui Yang,Zhiyang Gao, Zhili Zhang,Xuezhao Liu,Yang Li,Jing Xu,Yudong Jing,Shohei Mitani,Sudan He, Chonglin Yang. (2016). Negative regulation of phosphatidylinositol 3-phosphate levels in early-to-late endosome conversion.The Journal of Cell Biology 212(2),181-198.(In Focus: Making sure late endosomes are on time. Mitch Leslie. J Cell Biol 212: 127).

 

19. Meng Xu#,Yubing Liu,Liyuan Zhao,Qiwen Gan,Xiaochen Wang,and Chonglin Yang. (2014). The lysosomal cathepsin protease CPL-1 plays a leading role in phagosomal degradation of apoptotic cells in Caenorhabditis elegans. Molecular Biology of the Cell 25, 2071-2083.

 

20. Didi Chen#, Youli Jian#, Xuezhao Liu, Yuanya Zhang, Jingjing Liang, Xiaying Qi, Hongwei Du, Wei Zou, Lianwan Chen,Yongping Chai,Guangshuo Ou,Long Miao,Yingchun Wang, and Chonglin Yang. (2013). Clathrin and AP2 are required for phagocytic receptor-mediated apoptotic cell clearance in Caenorhabditis elegans. PLoS Genetics 9(5), e1003517.

 

21. Jing Xu,Xiaojuan Sun,Yudong Jing,Mo Wang,Kai Liu,Youli Jian,Mei Yang,Zhukuan Cheng,Chonglin Yang. (2012). MRG-1 is required for genomic integrity in Caenorhabditis elegans germ cells. Cell Research 22, 886-902.

 

22. Didi Chen#, Hui Xiao#, Kai Zhang, Bin Wang, Zhiyang Gao, Youli Jian, Xiaying Qi, Jianwei Sun, Long Miao, Chonglin Yang, 2010. Retromer Is Required for Apoptotic Cell Clearance by Phagocytic Receptor Recycling. Science, 3271261-1264.

 

23. Xiao Yang* and Chonglin Yang*. 2010. An everlasting role of animal models in understanding human disease. Journal of Genetics and Genomics 37: 557.

 

24. Youli Jian, Zhiyang Gao, Jianwei Sun, Qinfang Shen, Feng Feng, Yudong Jing and Chonglin Yang, 2009. RNA aptamers interfering with nucleophosmin oligomerization induce apoptosis in cancer cells, Oncogene. 28:4201-4211.

 

25. Qinfang Shen#, Fengsong Qin#, Zhiyang Gao#,Jie Cui,Hui Xiao,Zhiheng Xu,and Chonglin Yang, 2009.Adenine Nucleotide Translocator Cooperates with Core Cell Death Machinery to Promote Apoptosis in Caenorhabditis elegans. Molecular and Cellular Biology, 29(14): 3881–3893.

 

26. Mei Yang, Jianwei Sun, Xiaojuan Sun, Qinfang Shen,Zhiyang Gao,Chonglin Yang, 2009.Caenorhabditis elegans Protein Arginine Methyltransferase PRMT-5 Negatively Regulates DNA Damage-Induced Apoptosis. PLoS Genetics, 5(6): e1000514. doi:10.1371/journal.pgen.1000514.

 

27. Hui Xiao, Didi Chen, Zhou Fang, Xiaojuan Sun, Jing Xu, Song Song, Jiajia Liu and Chonglin Yang, 2009. Lysosome biogenesis mediated by vps-18 affects apoptotic cell degradation in Caenorhabditis elegans. Molecular Biology of the Cell, 20: 21-32.


28.Chonglin Yang, Myoung Sook Kim, Devulapalli Chakravarty,Fred E. Indig and France Carrier, 2009.Nucleolin Binds to the Proliferating Cell Nuclear Antigen and Inhibits Nucleotide Excision Repair. Molecular and Cellular Pharmacology, 1(3):130-137.

 

29. Xiaochen Wang, Jin Wang, Keiko Gengyo-Ando, Lichuan Gu, Chun-Ling Sun, Chonglin Yang, Yong Shi, Tetsuo Kobayashi, Yigong Shi, Shohei Mitani, Xiao-Song Xie & Ding Xue, 2007. C. elegans mitochondrial factor WAH-1 promotes phosphatidylserine externalization in apoptotic cells through phospholipid scramblase SCRM-1. Nature Cell Biology, 9:541-549.


30.Chonglin Yang, Nieng Yan, Jay Parish, Xiaochen Wang, Yigong Shi and Ding Xue2006. RNA aptamers targeting the cell death inhibitor CED-9 induce cell killing in C. elegans. Journal of Biological Chemistry, 281:9137-44.

 

   (#表示共同第一作者,*表示共同通讯作者 )